Journal of Visualized Protocols

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VIDEO SCRIPT FOLLOWING THE [Journal of Visualized Protocols] FORMAT

OVERVIEW:

  • Length: 10-15 minutes.

  • Language: Professional, clear, coherent.

  • Visuals: Clean, professional, focused on actions and results.

DETAILED SCRIPT STRUCTURE

PART 1: INTRODUCTION
(Duration: ~1-2 minutes)

  • Scene: Narrator (could be you or a team member) sitting in front of a neutral background, wearing a white lab coat, looking directly at the camera.

  • Voice-over Script:

    • "(Hello, my name is [Your Name], from [Your Lab/Institution].)"

    • "This video presents a protocol for [Briefly describe the objective of the method/experiment]. For example: 'isolating and culturing mesenchymal stem cells from human adipose tissue'."

    • "[Your Method's Name] is an important tool in the field of [Your research field, e.g., Tissue Engineering, Pharmacology, Cell Biology]."

    • "Although previous methods exist, they often face limitations such as [State 1-2 weaknesses of old methods, e.g., low yield, low purity, difficulty in reproducibility]. Our method addresses these issues by [State 1-2 advantages of the new method, e.g., providing higher yield, using less toxic chemicals, having standardized steps]."

    • "The primary goal of this video is to provide a visual, step-by-step guide so that any researcher can successfully implement this protocol in their own laboratory."

PART 2: PROCEDURE
(Duration: ~7-10 minutes) - THE MOST IMPORTANT PART

  • Filming Principle: Each critical step should be filmed from multiple angles (overview, close-up of hands, close-up of equipment). Use illustrative images or text/graphic overlays to indicate chemical names, equipment, or important points.

  • Detailed Script (Adjust according to your specific procedure):

Scene 2.1: Solution Preparation & Setup

  • Visuals: Close-ups of weighing chemicals, measuring solutions, filtering. Display chemical formulas or names on screen.

  • Voice-over: "First, prepare the tissue digestion enzyme solution by mixing [Chemical A Name] and [Chemical B Name] in PBS buffer. The solution is then sterilized by filtration through a 0.22 µm membrane filter."

Scene 2.2: Main Sample Handling Step 1

  • Visuals: Clear shots of using scissors and forceps to process the tissue sample. Overhead camera angle...

  • Voice-over: "Adipose tissue is collected with informed consent. Wash the tissue several times with PBS solution supplemented with antibiotics to remove blood and debris. Then, using sterile surgical scissors, mince the tissue into small pieces of approximately 1-2 mm³."

Scene 2.3: Processing & Cell Harvesting Step

  • Visuals: Scenes of adding the tissue to the enzyme solution, shaking in an incubator. Followed by filtration through a cell strainer and centrifugation.

  • Voice-over: "Incubate the tissue with the enzyme solution at 37°C for 45-60 minutes, agitating periodically. After digestion, the mixture is filtered through a 100 µm cell strainer to remove undigested debris. The filtrate is centrifuged at 500g for 10 minutes to pellet the cells."

Scene 2.4: Culturing & Preservation Step

  • Visuals: Scenes of aspirating the supernatant, resuspending the cell pellet in culture medium, and seeding into a petri dish. Display the date and culture conditions (37°C, 5% CO2) on screen.

  • Voice-over: "Resuspend the cell pellet in complete culture medium. Seed the cells into a culture dish and place in a 37°C incubator with 5% CO2. Change the medium after the first 48 hours and then every 3-4 days thereafter."

PART 3: REPRESENTATIVE RESULTS
(Duration: ~2-3 minutes)

  • Scene: Display high-quality images, charts, graphs.

  • Voice-over Script:

    • "Using this protocol, we obtained a significant number of cells with high viability exceeding 95%, as confirmed by Trypan Blue staining."

    • "(Show microscope image) Microscopy images show the typical morphology of mesenchymal stem cells: spindle-shaped, adherent."

    • "(Show flow cytometry plot) Flow cytometry analysis shows the cells are positive for characteristic surface markers such as CD73, CD90, and CD105, and negative for hematopoietic markers."

    • "(Show staining images) After differentiation, these cells are capable of differentiating into osteogenic, chondrogenic, and adipogenic lineages, as demonstrated by Alizarin Red, Alcian Blue, and Oil Red O staining, respectively."

    • "These results confirm the effectiveness and reliability of the protocol for isolating and characterizing mesenchymal stem cells from adipose tissue."

PART 4: DISCUSSION & CONCLUSION
(Duration: ~1 minute)

  • Scene: Return to the narrator as in the introduction.

  • Voice-over Script:

    • "In summary, we have presented a detailed and reproducible protocol for [Repeat the method name]."

    • "Key points for success include: [1] Initial tissue quality, [2] Optimal digestion time, and [3] Sterile culture conditions."

    • "This method can be applied to [Mention potential applications, e.g., regenerative medicine research, drug screening, disease modeling]."

    • "We believe this visual presentation will facilitate the widespread adoption and refinement of this technique within the scientific community. Thank you for watching."

IMPORTANT NOTES TO EMULATE JVP:

  1. Scientific Accuracy: All procedures, chemical names, concentrations, and times must be thoroughly checked and 100% accurate.

  2. High-Quality Visuals: Use a good quality camera. Lighting must be ample and clear. Background should be clean and non-distracting (typically white or gray).

  3. Clear Audio: Use a microphone close to the speaker to reduce background noise. Narration should be recorded in a quiet room.

  4. Equipment & Attire: All equipment must be clean, new-looking. The operator wears a white lab coat and gloves.

  5. Illustrative Graphics (Text/Animation Overlay): Use graphics to:

    • Display chemical names, concentrations.

    • Display technical parameters (centrifuge speed, temperature, time).

    • Use arrows to point to important details in the shot.

    • Label parts of complex equipment.

  6. Pacing: The video pace should be moderately fast. Cut out all unnecessary moments, waiting periods. Use time-lapse techniques for long processes (e.g., cell growth).